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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Mech Dev. 2007 Mar 12;124(6):409–415. doi: 10.1016/j.mod.2007.02.003

Fig. 4. Loss of dentinal tubules in Tgfbr2fl/fl;Wnt1-Cre tooth germ.

Fig. 4

(A,B) SEM analysis of wild type and Tgfbr2fl/fl;Wnt1-Cre teeth after 28 days cultivation under a kidney capsule. Dentinal tubules are clearly visible in the wild type dentin layer (arrowheads in A), but not in the Tgfbr2fl/fl;Wnt1-Cre dentin (B). The structure of the Tgfbr2fl/fl;Wnt1-Cre enamel is indistinguishable from wild type. Broken line; dentin-enamel junction, d; dentin, e; enamel. (C-J) Nestin immunohistochemistry in wild type and Tgfbr2fl/fl;Wnt1-Cre tooth germ at E18.5 (C-F) and after 14 days cultivation under kidney capsules (G-J). E, F, I, and J are enlarged views of the yellow dashed boxes in C, D, G, and H, respectively. Nestin expression is detectable in wild type odontoblasts at the tip of the cusp (E, arrow), but not in Tgfbr2fl/fl;Wnt1-Cre odontoblasts (F). After cultivation under the kidney capsule, nestin is highly expressed in odontoblasts and in the dentin matrix area in wild type transplants (G,I). In Tgfbr2fl/fl;Wnt1-Cre transplants, nestin was barely detectable in odontoblasts (H,J). Scale bars: 20 μm in A, B; 100 μm in C, D, G and H; 25 μm in E, F, I and H.