Figure 3.

H₂O₂-induced Mer phosphorylation leads to activation of downstream antiapoptotic pathways. (A) Serum-starved WT and KO peritoneal exudate mouse macrophages were treated with 400 μM H₂O₂ for 10 min. Whole cell lysates were evaluated by immunoblot with antibodies for total and pAkt, as well as total and pErk 1/2. pAkt (B) and pErk 1/2 (C) were quantified by densitometry. To reflect relative phosphorylation, the IDV of the phospho band was divided by the IDV of the total protein band, and WT H₂O₂ was normalized to 100% for each experiment. Data shown are the mean ± sem from three independent experiments.