Figure 5.

Mer signaling provides a functional survival advantage for macrophages exposed to H₂O₂. WT and Mer KO peritoneal exudate mouse macrophages were harvested and serum-starved for 2 h. Prior to stimulation with 400 μM H₂O₂, macrophages were incubated in serum-free media, with or without 200 nM MerFc or RetFc for 2 h as indicated. Cultures were treated with H₂O₂ for an additional 3 h and then stained with trypan blue and counted in triplicate to determine cell viability. Survival of WT-untreated cells was normalized to 100% for each experiment. Data shown are the mean ± sem from three independent experiments.