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Plasmid restriction analysis of the blaCMY-bearing plasmids in E. coli DH10B transformants corresponding to the clinical isolates. Lane M, molecular weight markers (λ DNA-HindIII digest [New England Biolabs, Ipswich, MA] plus GeneRuler 100-bp Plus DNA ladder [Fermentas, Glen Burnie, MD]); lane 1, 1285; lane 2, YD006; lane 3, 34943. The plasmids were extracted by the standard alkaline lysis method, digested with PstI (New England Biolabs), and electrophoresed in a 0.7% agarose gel.
