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Time course of product formation with the GlcNAc-anhydroMurNAc-tetrapeptide as the substrate. The substrate (27 μM) was incubated in the presence of 106 nM sAmiD. The graph highlights biphasic behavior characteristic of a burst kinetic. The slopes of the dotted and dashed lines represent the initial and stationary state rates, respectively, describing the burst phenomenon (see the text for details). The curve fitting (solid line) was obtained by using equation 1.
