TABLE 1.
Muropeptides and HPLC internal standards used in this study
| Muropeptide | Structure | Reference(s) or source | Internal standardb (source) |
|---|---|---|---|
| Dansylated lipid I or lipid I (l-[14C]Ala) | Undecaprenyl-pyrophospho-MurNAc-l-Ala-γ-d-Glu-mA2pm(ɛ-Dansyl)-d-Ala-d-Ala | 9 | |
| Dansylated lipid II | Undecaprenyl-pyrophospho-MurNAc-(l-Ala-γ-d-Glu-mA2pm(ɛ-Dansyl)-d-Ala-d-Ala)-GlcNAc | 9 | |
| UDP-MurNAc-tripeptide | UDP-MurNAc-l-Ala-γ-d-Glu-mA2pm | 1,6 | |
| GlcNAc-anhydroMurNAc-tetrapeptidea | GlcNAc-anhydroMurNAc-l-Ala-γ-d-Glu-mA2pm-d-Ala | 17 | Gly-Phe (Sigma) |
| MurNAc-pentapeptide | MurNAc-l-Ala-γ-d-Glu-mA2pm-d-Ala-d-Ala | 17 | Phe-Phe (Sigma) |
| MurNAc-tripeptide | MurNAc-l-Ala-γ-d-Glu-mA2pm | 17 | Gly-Phe (Sigma) |
| MurNAc-tripeptide (Lys) | MurNAc-l-Ala-γ-d-Glu-l-Lys | 17 | |
| MurNAc-dipeptide | MurNAc-l-Ala-d-Glu | 17 | Phe-Phe (Sigma) |
| G1050 | MurNAc-d-Ala-d-Glu | Bachem | Ala-Ala-Ala-Ala (Sigma) |
| G1060 | MurNAc-l-Ala-l-Glu | Bachem | Ala-Ala-Ala-Ala (Sigma) |
a
Also referred to in the literature as tracheal cytotoxin (2).
b
Internal standards used to improve the precision of the estimation of muropeptides hydrolysis followed by HPLC.