TABLE 1.
Primers used in this study
| Primer | Experiment | Sequence (5′ to 3′) | Gene |
|---|---|---|---|
| RTG-A | PCR | CTCACGCTATCATTAAATGC | blaRTG |
| RTG-B | PCR | TCAAACGAGGCGTCTGTCTCTG | blaRTG |
| RTG-4-T69S-A | Site-directed mutagenesis | CGTTTTCCTCTAAGTAGTACCTTTAAAACACTTGCC | |
| RTG-4-T69S-B | Site-directed mutagenesis | GGCAAGTGTTTTAAAGGTACTACTTAGAGGAAAACG | |
| RTG-4-A49V-A | Site-directed mutagenesis | GAATTGGTCTAGCTGTGCATGATTTGGAAACG | |
| RTG-4-A49V-B | Site-directed mutagenesis | CGTTTCCAAATCATGCACAGCTAGACCAATTC | |
| P13B | Gene rrn (16S rRNA) | CGGGATCCCAGGCCCGGGAAC | rRNA |
| UNI14 | Gene rrn (16S rRNA) | GTGCCAGCAGCCGCGGTAAT | rRNA |
| Gsp1 | 5′-RACE | TACAGGTGAGTATGTAACGAGATTGC | |
| Gsp2 | 5′-RACE | TTCCCGTTTCCAAATCATGCGCAGC | |
| Gsp3 | 5′-RACE | TTCAGCATTGGTAACCGCTTC |