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In vitro stability of anionic liposomes. Liposomes encapsulating PS-ODN833-PEI nanosized particles were incubated in PBS at 4°C, room temperature, and 37°C. At the times indicated, samples were collected and eluted on a Sephadex G-200 column, and then the concentrations of free and encapsulated PS-ODN833 were determined by UV spectrophotometry (A260). Data are shown as the means ± SD of results from three independent experiments. d, day.
