FIG. 3.

Effects of anti-mecA encapsulated PS-ODN833 on the growth of WHO-2 colonies. Liposome-encapsulated PS-ODN833 (L-ODN833) was added to cell cultures containing 1.5 × 10⁸ CFU/ml WHO-2 to a final concentration of 0.7, 2, 6, or 18 μM. Additional cell cultures were treated with free PBS in a volume equal to that of the encapsulated-PS-ODN833 preparation as a control, liposome-encapsulated PBS (L-PBS), 18 μM liposome-encapsulated mismatched PS-ODN (L-Mismatched), 0.2 μM free PEI (F-PEI), or 18 μM free PS-ODN833 (F-ODN). Aliquots of each culture were collected at 6 h, diluted, and inoculated onto solid agar containing 6 μg/ml of oxacillin. The number of CFU was calculated from the number of colonies growing on plates. The data are shown as means ± SD for 10 samples. ***, P < 0.01 for comparison to control values; ###, P < 0.01 for comparison to results for free PS-ODN833.