TABLE 1.
Bactericidal efficacy of MB-PAA-DNP against gram-positive and gram-negative bacteria
| Strain | Treatmenta | Viable bacteria (mean log10 CFU) ± SE at a light fluence (J/cm2)b of:
|
|||||
|---|---|---|---|---|---|---|---|
| 0 | 11.4 | 22.8 | 45.6 | 68.4 | 136.8 | ||
| S. aureus ATCC 25923 | L-N+ | 6.16 ± 0.04 | 6.14 ± 0.03 | 6.15 ± 0.07 | 6.15 ± 0.14 | 6.14 ± 0.08 | 6.12 ± 0.11 |
| L+N+ | 6.16 ± 0.04 | 6.13 ± 0.04 | 4.58 ± 0.13 | 3.38 ± 0.09 | 2.00 ± 0.10 | 1.95 ± 0.08 | |
| E. coli K-12 | L-N+ | 6.22 ± 0.03 | 6.20 ± 0.04 | 6.18 ± 0.06 | 6.16 ± 0.03 | 6.17 ± 0.04 | 6.15 ± 0.04 |
| L+N+ | 6.22 ± 0.03 | 6.05 ± 0.05 | 5.88 ± 0.08 | 4.52 ± 0.19 | 3.66 ± 0.09 | 3.27 ± 0.17 | |
| P. aeruginosa PAO1 | L-N+ | 6.15 ± 0.05 | 6.14 ± 0.02 | 6.14 ± 0.07 | 6.13 ± 0.07 | 6.10 ± 0.07 | 6.09 ± 0.11 |
| L+N+ | 6.15 ± 0.05 | 6.03 ± 0.07 | 5.83 ± 0.12 | 4.93 ± 0.07 | 3.86 ± 0.14 | 3.77 ± 0.09 | |
| Acinetobacter sp. strain AC481 | L-N+ | 6.25 ± 0.01 | 6.23 ± 0.01 | 6.20 ± 0.05 | 6.17 ± 0.05 | 6.17 ± 0.04 | 6.17 ± 0.07 |
| L+N+ | 6.16 ± 0.01 | 5.77 ± 0.03 | 5.20 ± 0.16 | 4.64 ± 0.07 | 4.17 ± 0.13 | 3.96 ± 0.10 | |
| Acinetobacter sp. strain AC811 | L-N+ | 6.15 ± 0.03 | 6.13 ± 0.03 | 6.15 ± 0.07 | 6.13 ± 0.11 | 6.10 ± 0.09 | 6.09 ± 0.09 |
| L+N+ | 6.15 ± 0.03 | 6.03 ± 0.04 | 5.84 ± 0.05 | 4.92 ± 0.07 | 4.09 ± 0.06 | 3.92 ± 0.09 | |
a
As described in Materials and Methods, L-N+ represents the bacterial suspensions incubated in the dark in the presence of MB-PAA-DNP, and L+N+ represents the bacterial suspensions exposed to red light in the presence of MB-PAA-DNP. The concentration of MB-PAA-DNP used in these experiments was 10 mg/ml.
b
Light irradiance used was 38 mW/cm2. Values are means of three determinations.