TABLE 1.
Oligonucleotides used for gene sequencing and evaluation of gene expression
| Gene name (gene product) | GenBank accession no. | Primer | Nucleotide sequence (5′ to 3′) | Nucleotide coordinatesa |
|---|---|---|---|---|
| ERG11 (lanosterol 14-α-demethylase) | L40389 | ERG11-1F | CTACAATCGAGTGAGCTTG | 17 to 35 |
| ERG11-1R | GTAGAACACAAGTGGTGG | 729 to 746 | ||
| ERG11-2F | GGTCGTTGAACTATTGGAG | 584 to 602 | ||
| ERG11-2R | GGACCCAAGTAGACAGTC | 863 to 880 | ||
| ERG11-3Fb | CCATCACATGGCAATTGC | 688 to 705 | ||
| ERG11-3Rb | GGTCATCTTAGTACCATCC | 1445 to 1463 | ||
| ERG11-4F | CGTGAGAAGAACGATATCC | 1380 to 1398 | ||
| ERG11-4R | CACCTTCAGTTGGGTAAC | 2047 to 2064 | ||
| ERG11-5F | CGCTTACTGTCAATTGGG | 1991 to 2008 | ||
| ERG11-5R | GTCATATGCTTGCACTGC | 2397 to 2414 | ||
| PDR1 (transcription factor involved | AY700584 | PDR1-Fb | TGGAAAAGCTTGTGATAGCTG | 81 to 101 |
| in pleiotropic drug resistance) | PDR1-Rb | TCTGATGACTGTAGTAGCCGA | 498 to 518 | |
| NC005967 | PDR1-SF | CGTTATTGAGAGAATATGCAA | 47542 to 47562 | |
| PDR1-SR | AGGCTATGCACACTGTCTAA | 50891 to 50910 | ||
| CDR1 (ABC transporter) | AF109723 | CDR1-Fb | GAAGTCTATGGAAGGTGC | 1084 to 1101 |
| CDR1-Rb | GTCTAGCGTAAGTCTCTC | 1383 to 1400 | ||
| CDR2 (ABC transporter) | AF251023 | CDR2-Fb | GTTGAGTACTGGCACAAC | 362 to 379 |
| CDR2-Rb | GATGGCAAAGAACATGGC | 695 to 712 | ||
| HSP12 (heat shock protein) | NC006033 | HSP12-Fb | ACTTGGAGACGTATTCGACGG | 391031 to 391051 |
| HSP12-Rb | TGTCTGACGCTGGTAGAAAGA | 391299 to 391308 | ||
| ACT (β-actin) | AF069746 | ACT-Fb | TATTGACAACGGTTCCGG | 949 to 966 |
| ACT-Rb | TAGAAAGTGTGATGCCAG | 1177 to 1194 |
a
Nucleotide coordinates refer to those for the corresponding gene sequence in the GenBank database.
b
Primers used for evaluation of gene expression.