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. 2009 May 1;191(14):4522–4533. doi: 10.1128/JB.00272-09

FIG. 7.

FIG. 7.

Orf4 protects cells from oxidative stress. (A) Constitutive expression of Orf4. Four B. cereus strains including the wild-type strain, WT708 (orf4-null mutant), WT710 (wild-type strain harboring pRF305), and WT711 (orf4-null mutant harboring pRF305) were measured for Orf4 expression by Western blotting in the absence of environmental stress. The constitutive Orf4 expressions in strain WT710 and WT711 are clearly shown. OD600, optical density at 600nm. (B) Orf4 overexpression increases cell viability under the condition of 50°C heat stress or 1.5 mM t-BOOH treatment. A cell culture was harvested every 30 min to measure cell viability by plating count. The diamond, square, asterisk, triangle, and circle symbols denote the survival rate of wild-type B. cereus, WT708, WT709 (orf4-null mutant harboring pRF304), WT710, and WT711, respectively. The means of three replications are displayed for cell viability at each recorded time point.