TABLE 1.
Bacteria strains and plasmids used in this study
| Strain or plasmid | Genotype and/or description | Source or reference |
|---|---|---|
| B. cereus strains | ||
| ATCC 14579 | Wild type | ATCC Biological Resource Center |
| WT708 | Δorf4 mutant | This work |
| WT709 | Δorf4-pRF304 orf4 complementary strain | This work |
| WT710 | Wild-type-pRF305 orf4 overexpression strain | This work |
| WT711 | Δorf4-pRF305 orf4 overexpression strain | This work |
| E. coli strains | ||
| DH5α | General-purpose cloning | Invitrogen |
| BL21(DE3) | For protein expression | Novagen |
| Plasmids | ||
| pMAD | ermC bgaB | 8 |
| pMAD-B-S-Y | sigB anti-spc rsbY | This work |
| pHY300PLK | Tcr | Takara |
| pRF304 | pHY300PLK-orf4, Tcr, orf4 original promoter | This work |
| pRF305 | pHY300PLK-orf4, Tcr, orf4 tufA promoter | This work |
| pDG1728 | Spr | Bacillus Genetic Stock Center |
| pET11-a | E. coli overexpression vector, Apr | Novagen |
| pET14-b | E. coli overexpression vector, Apr | Novagen |
| pET14-b-orf4 | orf4 | This work |
| pGEX-6p-3 | For DNA assays | GE Healthcare Biosciences |