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Production of 1-methylxanthine (⧫) and 3-methylxanthine (×) from theophylline by cell extracts prepared from P. putida CBB5 grown in soytone-supplemented M9-theophylline medium. After 5 h, 0.15 mM NADH was added to the enzyme reaction mixture (indicated by an arrow). Addition of NADPH at the same time also enhanced 1- and 3-methyxanthine production (data not shown).
