TABLE 1.
Location and identities of the mutation in the yjbM and ywaC
| Mutant | Position of mutationa | Amino acid substitution or description of mutation |
|---|---|---|
| yjbM mutants | ||
| yjbM4 | 316G → T | Asp106 → Tyr |
| yjbM8 | 241C → T | Gln81 → stop codon |
| yjbM27, -28 | 415G → T | Glu139 → stop codon |
| Δ(424A—485G) | 62-bp deletion | |
| yjbM31, -33, -34, -35 | Δ(363T—382C) | 20-bp deletion |
| yjbM32 | 368T → A | Val123 → Glu |
| yjbM43 | 319T → A | Tyr107 → Asn |
| yjbM44 | 283G → A | Ala95 → Thr |
| 460G → A | Glu154 → Lys | |
| yjbM46 | 514A → G | Arg172 → Gly |
| yjbM47 | Δ29T | 1-bp deletion |
| yjbM49 | Δ(462A—472A) | 11-bp deletion |
| yjbM50 | 239G → A | Cys80 → Tyr |
| yjbM52 | 29T → A | Leu10 → stop codon |
| ywaC mutants | ||
| ywaC1,- 2, -5, -6, -7, -11, -25 | 631T → A | Stop codon 210 → Lys |
| ywaC3 | 3G → T | Stop codon → Lys |
| ywaC9 | 575G → T | Ala192 → Val |
| ywaC10 | A511-514 → addition of A | Frameshift |
| ywaC12 | Addition of GCGCTCGAT after T90 | 9-bp addition |
| ywaC22 | 197A → T | Lys60 → Ile |
| ywaC23 | 328C → T | Gln110 → stop codon |
| ywaC24 | Addition of CC after A437 | Frameshift |
| ywaC26 | 572C → A | Ala191 → Asp |
| ywaC30 | 527T → A | Leu176 → stop codon |
| ywaC37 | 149A → CC | Frameshift |
| ywaC39 | 268G → C | Gly90 → Arg |
| ywaC40 | 575C → A | Ala192 → Glu |
a
Numbering from the start codon (ATG) of the open reading frame.