FIG. 4.

Effect of glucose on cry4A promoter activity in vivo during different growth phases. cry4A promoter-lacZ fusion variants were grown in G medium (8 h for pre-stationary phase, 14 h for stationary phase, and 18 h for post-stationary phase) and resuspended in RM (upper panel) with glucose (gray bars) or without glucose (white bars). β-Galactosidase activities were measured at 8 h after resuspension. The hatched bars (T₀) show β-galactosidase activities at the time of resuspension in RM. WT, wild type. The repression index (lower panel) was calculated as the ratio of β-galactosidase activity obtained in the absence of glucose to that in the presence of glucose. Subscripts show nucleotide positions relative to +1, and substitutions are shown with arrows. Values are the average of three independent experiments, each assayed in duplicate. Error bars represent the standard deviation. M.U., Miller units.