TABLE 1.
Strains and plasmids used in this study
| Plasmid or strain | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Plasmids | ||
| pGEM-T Easy | Ampr, E. coli cloning vector | Promega |
| pHT370 | Ampr Eryr, shuttle vector | Pasteur Institute |
| pSK1 | pGEM-T Easy containing cry4A coding sequence with native promoter | This work |
| pSK2 | pHT370 containing cry4A coding sequence with native promoter | This work |
| pSK3 | pGEM-T Easy containing 117-bp cre1-2 | This work |
| pSK4 | pGEM-T Easy containing cre1-2 with mutation (G49→A) in cre1 | This work |
| pSK5 | pGEM-T Easy containing 458-bp cry4A promoter | This work |
| pSK6 | pGEM-T Easy containing 3,067-bp lacZ | This work |
| pSK7 | pGEM-T Easy containing cry4Apro-lacZ fusion | This work |
| pSK8 | pHT370 containing cry4Apro-lacZ fusion | This work |
| pSK9 | pHT370 with cry4A coding sequence with G49→A in cre1 | This work |
| Strains | ||
| 4Q7 | B. thuringiensis subsp. israelensis plasmidless mutant | BGSCa |
| SK2 | 4Q7 containing pSK2 | This work |
| SK9 | 4Q7 containing pSK9 [(G49→A) mutation in cre1 in pSK2] | This work |
| SK10 | 4Q7 containing pSK8 | This work |
| SK11 | 4Q7 containing pSK11 [(A36→C) in pSK8]b | This work |
| SK12 | 4Q7 containing pSK12 [(C37→A) in pSK8]b | This work |
| SK13 | 4Q7 containing pSK13 [(T40→C) in pSK8]b | This work |
| SK14 | 4Q7 containing pSK14 [(A43→G) in pSK8]b | This work |
| SK15 | 4Q7 containing pSK15 [(A44→C) in pSK8]b | This work |
| SK16 | 4Q7 containing pSK16 [(A47→C) in pSK8]b | This work |
| SK17 | 4Q7 containing pSK17 [(A48→C) in pSK8]b | This work |
| SK18 | 4Q7 containing pSK18 [(G49→A) in pSK8]b | This work |
| SK19 | 4Q7 containing pSK19 [(G49→T) in pSK8]b | This work |
| SK20 | 4Q7 containing pSK20 [(G49→C) in pSK8]b | This work |
a
BGSC, Bacillus Genetic Stock Center (Ohio State University, Columbus).
b
Point mutations are done in the cre1 region of the promoter.