FIG. 5.

Phylogenetic tree of 3-hydroxypropionyl-CoA dehydratase. The tree is based on amino acid sequence analysis and rooted with propionyl-CoA synthase from C. aurantiacus. Tree topography and evolutionary distances are given by the neighbor-joining method with Poisson correction. The scale bar represents a difference of 0.1 substitutions per site. Numbers at nodes indicate the percentage bootstrap values for the clade of this group in 1,000 replications. The enzyme groups from the order Sulfolobales and from Clostridia are indicated. Fusion refers to the dehydratase domain of an archaeal fusion enzyme that contains an enoyl-CoA hydratase fused to a 3-hydroxyacyl-CoA dehydrogenase.