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. 2009 May 11;29(14):3817–3831. doi: 10.1128/MCB.00243-09

FIG. 12.

FIG. 12.

Retention of either the DNA-binding domain or SID of Cfp1 is required to rescue in vitro differentiation. (A) Colony morphology following induction of differentiation. CXXC1+/+, CXXC1−/−, and CXXC1−/− ES cells expressing full-length Cfp1 (1-656) or the indicated Cfp1 truncations or mutations (or carrying the empty expression vector) were cultured in the absence of LIF for 10 days. Magnification, ×10. (B) Cells were grown as in described for panel A and then disaggregated with trypsin, reseeded into gelatin-coated culture dishes, and stained for alkaline phosphatase activity using a leukocyte detection kit. Numbers represent percentages of cells positive for alkaline phosphatase activity. At least 200 cells were scored for each clone. Magnification, ×10.