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. 2009 May 11;29(14):3817–3831. doi: 10.1128/MCB.00243-09

FIG. 13.

FIG. 13.

Retention of either the DNA-binding domain or SID of Cfp1 is required to rescue in vitro differentiation of CXXC1−/− ES cells. CXXC1+/+, CXXC1−/−, and CXXC1−/− ES cells expressing full-length Cfp1 (1-656) or the indicated Cfp1 truncations or mutations (or carrying the empty expression vector) were cultured in the absence of LIF to induce differentiation. Cells were collected at day 0 (undifferentiated) and day 10 after induction of differentiation. Total RNA was isolated, and RT-PCR was performed to analyze the expression of Oct4, a marker of undifferentiated ES cells, along with the following lineage and developmentally specific markers: Brachyury (mesoderm), β-H1 (primitive erythroid), c-fms (myeloid), gp-IIB (megakaryocyte), Gata-4 (endoderm), and Hprt, a housekeeping gene that serves as a control for cDNA quantity and integrity.