FIG. 2.
Cfp1 contains redundant functional domains sufficient to rescue the deficiency in plating efficiency observed in CXXC1−/− ES cells. The graph summarizes the plating efficiency of CXXC1+/+, CXXC1−/−, and CXXC1−/− ES cells expressing full-length Cfp1 (1-656) or the indicated Cfp1 fragments (or carrying the empty expression vector). Plating efficiency was determined by counting the number of colonies formed after plating 400 cells. The graph summarizes data from at least three independent experiments performed in triplicate, and error bars represent standard error. Asterisks denote statistically significant (P < 0.05) differences compared to CXXC1−/− ES cells expressing Cfp11-656.