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. 2009 May 11;29(14):3817–3831. doi: 10.1128/MCB.00243-09

FIG. 8.

FIG. 8.

Isolation of CXXC1−/− ES cell clones expressing Cfp1 fragments or point mutations. (A) A schematic of each Cfp1 construct is presented. The approximate location of the C169A and C375A mutations within the CXXC domain and SID, respectively, are denoted by X. (B) The level of Cfp1 expression in CXXC1+/+, CXXC1−/−, and CXXC1−/− ES cells expressing either full-length Cfp1 (1-656) or the indicated Cfp1 truncations or point mutations (or carrying the empty expression vector) was assessed by Western analysis using antiserum directed against Cfp1 (25). A representative blot is presented. Asterisks denote the position of each FLAG-Cfp1 protein. The blot was reprobed for β-actin as a loading control. The graph summarizes the results from at least three independent experiments, and error bars represent standard error.