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. 2009 May 18;29(14):3941–3952. doi: 10.1128/MCB.00384-09

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Copyright © 2009, American Society for Microbiology

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FIG. 1. — Identification of YNL187 by a bypass genetic screen. (A) Strategy for isolating prp28Δ bypass suppressors. See the text for details. (B) Restriction maps of the chromosomal PRP28, prp28Δ::TRP1 (TRP1), and the plasmid (URA3/ADE2-marked)-borne PRP28. (C) PRP28 is not present in the bypass suppressor strains. DNAs isolated from bypass suppressor candidates (lanes 3 to 7) were digested with HindIII and probed for the presence of the chromosomal prp28Δ::TRP1 and the plasmid-borne PRP28. The probe hybridizes to a 2.9-kb DNA band corresponding to the chromosomal PRP28 in the wild-type strain (lane 1), which is replaced by an 0.4-kb band corresponding to prp28Δ::TRP1 and by an additional 2.2-kb band corresponding to the plasmid-borne PRP28 in the starting strain (lane 2). Only the 0.4-kb band is detected in the five bypass suppressors (lanes 3 to 7). The star indicates a band with a cross-reaction induced by the probe.