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. 2009 Apr 29;83(14):6995–7003. doi: 10.1128/JVI.00268-09

FIG. 8.

FIG. 8.

Detection of XMRV RNA in prostatic secretions of prostate cancer patients. (A) Locations of qRT-PCR primers and probe (qF, qR, and qP for forward primer, reverse primer, and probe, respectively) and two rounds of nested PCR primers (nA and nB for first round; nC and nD for second round) in the variable regions of the XMRV gp70 env gene. qRT-PCR amplifies a region of 74 nucleotides (nt) between variable C and B regions (VRC and VRB); second-round nested RT-PCR amplifies a region of 218 nt including both the variable A region (VRA) and VRC. (B) qRT-PCR results for RNA isolated from EPS or standard control 1.95-kb env RNA (circles). Ct, cycle threshold. (C) Nested RT-PCR for env amplicon “a” of total RNA isolated from EPS. As a control, XMRV env RNA was used (lane 1). The case (VP) numbers are indicated, and the XMRV-positive cases are shown in red.