FIG. 3.

Nuclear-cytoplasmic shuttling of EBNA-LP in heterokaryons between human B cells and mouse 3T3 cells. (A, D, and G) X50-7 cells were fused with murine 3T3 cells and stained with EBNA-LP, EBNA2, or hnRNPC antibodies as indicated. (B, E, and H) Human and mouse cell nuclei (H and M, respectively) were distinguished by staining them with Hoechst stain, which gives a diffuse staining pattern for human nuclei and diffuse/punctate staining for mouse nuclei. (C, F, and I) Heterokaryon formation was confirmed by differential interference contrast.