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. 2009 Apr 29;83(14):7109–7116. doi: 10.1128/JVI.00654-09

FIG. 7.

FIG. 7.

Interaction of an EBNA-LP coactivation mutant with HDAC4. (A) Immunoprecipitation (IP) assay with 293 cells cotransfected with HDAC4 and wild-type (WT) or coactivation mutant EBNA-LP or an EBNA-LP with seven W repeats. Cell extracts were precipitated with an anti-Flag monoclonal antibody that reacts with Flag epitope-tagged EBNA-LP (+) or no antibody (−), followed by Western blot analysis with an HDAC4 monoclonal antibody. Molecular weights are shown on the left. (B) Western blot analysis of cell extracts used for the immunoprecipitations in panel A. The expression levels of EBNA-LP (top) and HDAC4 (bottom) in each of the three cotransfections are shown. Because the anti-EBNA-LP antibody reacts with the W repeats, the expression level of the 62-kDa EBNA-LP relative to the smaller isoforms is exaggerated. Molecular weights are shown on the left.