FIG. 1.

Paramyxoviruses differ from one another in the requirements for VLP production. 293T cells were transfected to produce the indicated viral proteins. Radiolabeled viral proteins from cell lysates were collected by immunoprecipitation. Particles from culture supernatants were purified by centrifugation through sucrose cushions and flotation on sucrose gradients. Viral proteins from both cell lysates and purified particles were fractionated by SDS-PAGE and detected using a phosphorimager. The result shown is a representative example of three independent experiments.