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Mumps VLPs produced from transfected cells resemble MuV virions morphologically. 293T cells were infected with MuV strain 88-1961 or were transfected to produce the indicated MuV proteins for VLP production. Virions/VLPs were purified by centrifugation through sucrose cushions and flotation on sucrose gradients as described in Materials and Methods. Virions/VLPs were adsorbed onto carbon-coated grids and visualized by transmission electron microscopy after negative staining.
