FIG. 6.

Production of PIV5-like particles with MuV glycoproteins and production of mumps VLPs with PIV5 glycoproteins. 293T cells were transfected to produce the indicated viral proteins for VLP production. Plasmid quantities are as indicated in Materials and Methods, with the exception of the increasing HN and F plasmid amounts, which represent 0.75, 1.5, and 3.0 μg of plasmid DNA. VLP production efficiency was measured, and values were normalized to the maximum value obtained in each experiment. Each result shown is representative of at least two independent experiments.