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Nef- and PMA-induced CD4 downregulation analysis in lymphoid cell lines expressing CD4 mutants. A2.01 cell lines stably expressing WT CD4, CD4 S/A, or CD4 CC/SS were transduced to achieve Nef expression or treated with PMA prior to analysis of cell surface CD4 levels (A) and internalization (B), as described in the legend to Fig. 1. Values are the means of three independent experiments; error bars represent 1 standard deviation from the mean.
