FIG. 3.

Migrating cDC have a more mature phenotype than do LN-resident cDC. (A) At 48 h after primary RSV infection, MLN cDC were stained with anti-CD40, -CD80, and -CD86. Based on CFSE staining, the cDC were divided into LN-resident cDC (filled histogram) and migrating cDC (open histogram). (B) At several time points after primary RSV infection, the levels of expression of CD40, CD80, and CD86 on LN-resident cDC and migrating cDC were determined. The experiment was performed twice with five mice per time point. Average mean fluorescence intensities (MFI) are depicted. Error bars represent SEM.