Layout of the chamber and an example of method and laboratory sample assignment. The large (929 cm2) and small (103 cm2) squares indicate the locations of the test coupons (stainless steel or carpet). The circles (60 cm2) indicate the reference agar plate (A) locations. For each chamber run, the method (S, swab; W, wipe, and V, vacuum) and laboratory (1, 2, and 3) were randomly assigned to each square test coupon as described in the text.