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. 2009 May 8;75(13):4297–4306. doi: 10.1128/AEM.02549-08

TABLE 4.

Results of repeated sampling passes and final wash solution by surface material and sampling method

Surface Parameter Result for:
Swab
Wipe
Vacuum
Mean CV Mean CV Mean CV
Steel No. of reference plates 16 16 16
Reference agar (CFU/100 cm2)a NAd 250 22 NA
No. of samplesb 8 9 5
100% × P1/(P1 + P2 + P3 + W)c 87 11 95 2.9 88 2.8
100% × P2/(P1 + P2 + P3+ W) 5.4 68 2.7 68 5.3 34
100% × P3/(P1 + P2 + P3+ W) 4.6 170 1.9 83 1.2 61
100% × W/(P1 + P2 + P3+ W) 2.7 130 0.3 110 5.4 31
100% × (P1 + P2 + P3 + W)/A NA 20 48 NA
Carpet No. of reference plates 12 12 12
Reference agar (CFU/100 cm2) 250 15 250 15 250 15
No. of samples 8 6 6
100% × P1/(P1 + P2 + P3 + W) 8.9 37 20 15 26 64
100% × P2/(P1 + P2 + P3 + W) 2.9 61 9.4 10 2.7 61
100% × P3/(P1 + P2 + P3 + W) 2.7 33 6.6 28 1.4 130
100% × W/(P1 + P2 + P3 + W) 86 5.7 64 4.5 70 26
100% × (P1 + P2+ P3 + W)/A 112 19 63 9.6 13 66
a

Reference agar samples for steel-swab and steel-vacuum combinations were not available for the repeated sampling pass experiment.

b

Steel-wipe combination results exclude a single sample because the sample was lost. Steel-vacuum combination results exclude a single sample because the tool to move the shelves was dropped, resulting in one of the samples not being opened prior to settling.

c

Pi is the result from sampling pass i (where i = 1, 2, or 3); W is the result from the final wash solution; and A is the mean agar result for the run.

d

NA, not applicable.