Figure 3.

Ten chromosome 2 deletions of seven different size classes were recovered. Verification and mapping of the deletions was performed by analysis of marker loss in cell line DNA. Because the deletions were made in an ES cell line derived from an F₁ hybrid embryo (129S1 × Cast/Ei), deletions could be mapped with polymorphic markers. (A) The 129S1 allele of D2Wsu32e was lost (−) in six of the 10 lines, indicating deletions on the 129S1 chromosome 2 of these cell lines. (B) The results for markers in the vicinity of Notch1 in the 10 cell lines are summarized and the deletions are arranged from largest to smallest. Note that only 129S1 alleles were lost. The vertical lines in B separate bins of markers. Markers within bins are not ordered relative to each other within the bins.