Figure 4.

Most deletion chromosomes do not have secondary rearrangements. Verification of cytology and mapping of deletions was performed by FISH with YAC probes to chromosome 2. Hybridization signals on chromosome 2 are indicated with arrows; signals without arrows are on chromosomes other than 2 and are caused by YAC chimerism. (A) Deletion line 3 has two chromosomes 2, both with signals for each of YACs 144F11 (green, 9 cM), 158D10 (red, 15 cM), and 188A3 (yellow, 98 cM). (B) Deletion line 3 has signal for YAC 188A3 (green, 98 cM) on both chromosomes 2, but is missing signal for 456A8 (red, 14 cM) on one. (C) Deletion line 3 again has two signals for 188A3 (green, 98 cM), but only one chromosome 2 signal for YAC 304E7 (red, 13 cM). (D) In deletion line 10, one chromosome 2 has an inverted duplication containing 406F11 (red, 38 cM) and 88C11 (green, 48 cM).