Carbon balance for a Pseudomonas sp. strain CT07 biofilm over an 8-day cultivation. Shown are cumulative CO2 exiting in gas phase (A), cumulative CO2 exiting in liquid phase (B), carbon remaining behind in the biofilm (C), cumulative carbon exiting as cells (D), cumulative carbon exiting as aggregates (less than 1% of vol) (E), and cumulative unused citrate (F). The carbon balance typically closed within 2 to 4%.