TABLE 1.

Absolute concentrations of IPL- and CL-derived GDGTs for all hot spring and soil samples, IPL-estimated archaeal cell densities, and archaeal 16S rRNA and amoA gene copy numbers (for Leonard's spring only)

Site	Sample	Distance from spring (cm)	Temp (°C)	pHa	Concn of GDGTs (ng/g dry sample)b																Estimated amt of archaeal cells/g	No. of archaeal 16S copies/g sediment	No. of amoA copies/g sediment
					IPL								CL
					0	1	2	3	4	Cren	Cren′	Total	0	1	2	3	4	Cren	Cren′	Total
Leonard's	Hot spring	0	58	8.4	1.1	0.4	1.6	0.4	1.1	5.8	0.6	11	0.1	0.0	0.0	0.0	0.0	0.2	0.0	0.4	1.1E+07	7.3E+07	5.3E+05
	Soil	10	36	8.6	9.7	18*	17*	1.9	2.1	8.2	0.6	22	4.4	0.9	0.8	0.7	1.2	5.8	0.2	14	5.7E+07	1.8E+09	7.1E+06
	Soil	30	28	8.4	1.3	6.6	4.4	0.8	NA	15	3.4	32	0.1	0.1	0.1	0.0	NA	0.0	0.0	0.5	3.2E+07	3.6E+07	1.6E+07
	Soil	60	20	8.6	0.8	4.0	2.3	0.4	NA	6.0	2.1	16	0.2	0.2	0.1	0.1	NA	2.9	0.3	3.9	1.6E+07	5.2E+07	1.8E+06
	Soil	150	14	8.6	2.8	1.8	1.2	0.6	NA	7.0	2.4	16	1.4	0.1	0.1	0.1	NA	1.3	0.3	3.2	1.6E+07	6.5E+07	7.3E+06
“Ray's”	Hot spring	0	89	8.2	42	48	75	130	210	22	7.5	540	5.4	4.6	6.8	11	13	2.1	0.4	44	5.4E+08	NA	NA
	Soil	10	42	7.0	4.8	0.9	1.8	1.9	7.6	20	2.9	40	2.0	0.8	2.7	2.2	5.8	16	0.9	31	4.0E+07	NA	NA
	Soil	20	33	6.1	2.2	2.5	3.9	6.5	NA	4.7	0.8	21	0.2	0.1	0.3	0.2	NA	3.5	0.3	4.5	2.1E+07	NA	NA
	Soil	40	25	6.9	0.4	0.2	0.4	0.3	NA	2.8	0.4	4.6	0.2	0.1	0.2	0.1	NA	0.8	0.1	1.4	4.6E+06	NA	NA
	Soil	80	20	7.0	1.6	1.1	0.7	0.3	NA	4.0	0.0	7.7	0.2	0.1	0.1	0.1	NA	1.3	0.2	1.9	7.7E+06	NA	NA
	Soil	250	12	7.7	0.2	0.0	0.1	0.1	NA	0.8	0.1	1.4	0.1	0.0	0.0	0.0	NA	0.2	0.0	0.5	1.4E+06	NA	NA

^apH was of streamers near source sediment sampled from “Ray's” hot spring.

^bCren, crenarchaeol; Cren′, regioisomer of crenarchaeol; NA, not analyzed. *, GDGTs 1 and 2 in Leonard's 10-cm soil are leading regioisomers (Fig. 2e and g). Numbers correspond to GDGT structures shown in Fig. 1 (e.g., “0” is GDGT-0).