FIG. 5.

Potassium release from Lactococcus lactis HP whole cells induced by lacticin 3147 in the absence (A) and presence (B) of 10 mM EGTA. Lacticin 3147 A1/A2 (1:1 molar ratio) was added simultaneously at a concentration of 1 μM each to whole cells (squares). Cells were preincubated with the lacticin A1 peptide for 3 min prior to a washing step, followed by the addition (black arrow) of lacticin A2 (triangles). In the control experiment, both lacticin 3147 peptides were added directly to washed cells (black line). One representative experiment is shown.