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Lacticin 3147 and nisin-induced CF-release from liposomes made of DOPC and DOPC supplemented with 0.1 mol% lipid II (LII). Peptides were added at a concentration of 0.25 μM to liposomes (25 μM phospholipid on a phosphorous basis) in buffer (gray bars), buffer with 1.25 mM CaCl2 (white bars) and buffer with 10 mM EGTA (black bars). Marker release was determined 2.5 min after peptide addition. Mean values of three independent experiments are shown.
