TABLE 2.
Primers used in this study
| Primer | Sequencea | Construct(s) | Use |
|---|---|---|---|
| OriVR | 5′-gcTCTAGAAGCTTATTAAGGGTTTTG-3′ | pSD5MR, pSD5V | Replication and oriV assays |
| RepAC | 5′-gcTCTAGATTAATTGAATAAGTTCGC-3′ | pSD5MR | Replication assay |
| OriVF | 5′-gcTCTAGAGCTCGTTTCTTTAGTGC-3′ | pSD5V | oriV assay |
| OriTF | 5′-gcTCTAGAGTTCTTGTATCACCAGCC-3′ | pSD5T | oriT assay |
| OriTR | 5′-gcTCTAGACTTTTTTATTGCTGATGG-3′ | ||
| RepAN | 5′-ccgcGGATCCATGGAGTACCCTATTTTC-3′ | pQERA | Promoter assay |
| RepAC | 5′-gccccAAGCTTAATTGAATAAGTTCGC-3′ | ||
| ParSR | 5′-AGAATGACCTTGTACCC-3′ | Copy no. estimation | |
| ParAN | 5′-ATGATAATTTCATTCC-3′ | ||
| PF | 5′-CAAAATCGCTAATAGAGGGTC-3′ | pBTK445 detection | |
| PR | 5′- GATACCGGAACCGCAACAC-3′ | ||
| TB11F1 | 5′-CCAATCACCCTAACCATGTC-3′ | Arbitrary PCR | |
| TB11F2 | 5′-AATCGCTCATTGCTGCTTGC-3′ | ||
| Arbit1 | 5′-GGCCACGCGTCGACTAGTCANNNNNN-3′ | Arbitrary PCR | |
| Arbit2 | 5′-GGCCACGCGTCGACTAGTCA-3′ | ||
| SoxBF | 5′-CAAGGGAAAAACACTGGTGAC-3′ | Copy no. estimation | |
| SoxBR | 5′-CCCTTTACGCCCCCTTTATC-3′ | ||
| MocoF | 5′-ctagTCTAGAGTGTACCTTCGGGTAC-3′ | pBTKSM | Complementation |
| MocoR | 5′-ctagTCTAGATGAATCCCTTGCTGCG-3′ |
a
Underlined sequences indicate restriction sites; nucleotides shown in lowercase were added to the 5′ ends of the sites to ensure complete digestion.