TABLE 3.

Predicted ORFs found in the partially sequenced genome of pBTK445

Position (start-stop)a	ORF name	No. of amino acids	Nearest homologous proteinb	Functional notes	% Amino acid identity with nearest phylogenetic relative	Nearest phylogenetic relative (GenBank accession no.)
1-613	orf1	Partial
1,101-631	orf2	156
1416-1159	orf3	85
2064-1420	orf4/parA	214	ParA	ATPase involved in plasmid partitioning	46	Chlorobium limicola plasmid pCL1 (NP_052163)
2981-2220	orf5/repA	253	RepA (belonging to RPA superfamily)	Plasmid replication initiator protein	52	Rhodospirillum rubrum genome (YP_425444)
5369-4053	orf6	438	DNA-directed DNA polymerase; subunit UmuC	Error-prone DNA repair	49	Methylibium petroleiphilum PM1 plasmid RPME01 (YP_001023066)
5749-5321	orf7	142	DNA-directed DNA polymerase; subunit UmuD	Error-prone DNA repair	57	Methylibium petroleiphilum PM1 plasmid RPME01 (YP_001023062)
6283-5858	orf8	141
7046-7399	orf9	117
7711-8076	orf10	121	Putative transcriptional regulator		32	Shigella boydii BS512 (ZP_00699160)
8838-8599	orf11	79	Prophage-related conserved transcriptional repressor protein		47	Xylella fastidiosa 9a5c (NP_297791)
8951-9298	orf12	115	Putative phage repressor		30	Bordetella avium (CAJ48906)
10371-22972	tagB locus (orf13 to orf25)		T4SS homologs	Conjugal transfer and type 4 secretion	See Table 4
23070-23393	orf26/ssb	107	ssDNA-binding protein	Controls activity of RecBCD nuclease	47	Salmonella enterica subsp. enterica (ZP_02353510)
23548-24537	virD4	Partial	TraN protein	VirD4-like protein	47	Plasmid pIPO2T (NP_444530)

^aAscending or descending nucleotide positions indicate that the gene is located on the positive or negative strand, respectively.

^bssDNA, single-stranded DNA.