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SDS-PAGE analysis of purified BoNT/A. A BoNT/A sample (5 μl) was electrophoresed under nonreducing conditions on a 10% polyacrylamide gel in parallel with BSA standards. After Coomassie brilliant blue staining, purified BoNT/A migrating as a single 150-kDa band was quantified by densitometric analysis against the BSA standards. Lane 1 shows the molecular mass standards, while lane 2 contains BoNT/A. Lanes 3 to 5 were loaded with the BSA standards (2, 4, and 6 μg, respectively).
