FIG. 2.
Comparative hydrolysis of peptides 7 and 8. BoNT/A (150 kDa; 50 ng/ml) was added to peptide substrates 7 and 8 (20 μM) in 100 μl of reaction buffer (HEPES, 20 mM, pH 7.4; ZnSO4, 200 μM; DTT, 5 mM; BSA 1 mg/ml) and incubated at 37°C for 90 and 180 min, times at which the fluorescent signal was monitored. The bar graph shows the mean deltas of fluorescence (total fluorescence − fluorescence of the peptide substrate alone in buffer) measured with peptide 7 (gray bars) and peptide 8 (black bars).