TABLE 2.
Peptide | Km (μM) | kcat (s−1) | kcat/Km (M−1 s−1) |
---|---|---|---|
8 | 13 ± 3 | 0.79 ± 0.06 | 6.1 (±0.5) × 104 |
9 | 11 ± 1 | 1.01 ± 0.04 | 9.2 (±0.4) × 104 |
10 | 0.79 ± 0.20 | 2.08 ± 0.12 | 2.63 (±0.15) × 106 |
11 | 0.13 ± 0.02 | 1.15 ± 0.05 | 8.85 (±0.38) × 106 |
Kinetic parameters were obtained under the conditions described in Materials and Methods. The plotted Km results for peptides 9, 10 (PL50), and 11 (PL51) are represented in Fig. S2 in the supplemental material.