TABLE 1.
Detection of GII.4 2006b/DenHaag NoV in buffer and various food products using A-, B-, H(2)-, and H(3)-conjugated magnetic beads with two automated extraction systemsa
| System | Matrix | No. of positive replicates/total no. at input virus titerb of:
|
|||
|---|---|---|---|---|---|
| 104 | 103 | 102 | 101 | ||
| iCropTheBug | Buffer | 3/3 | 2/3 | 1/3 | — |
| Lettuce | 3/3 | 1/3 | 0/3 | — | |
| Green onions | — | 2/3 | 1/3 | 0/3 | |
| Strawberries | 1/3 | 0/3 | 0/3 | — | |
| Deli ham | 2/3 | 1/3 | 0/3 | — | |
| Pathatrix | Buffer | — | 3/3 | 1/3 | 0/3 |
| Lettuce | — | 1/3 | 1/3 | 1/3 | |
| Green onions | — | 1/3 | 1/3 | 1/3 | |
| Strawberries | — | 0/3 | 0/3 | 1/3 | |
| Deli ham | — | 0/3 | 0/3 | 0/3 | |
a
For each data set, the three viral additions were undiluted, diluted at 1/10, and diluted at 1/100.
b
The input titer was calculated for each experiment and varied due to differences in the stock sample. The quantity of input virus has been rounded to the nearest factor of 10 copies/250 ml. —, dilution not tested.