TABLE 2.
Detection of NoV strains in buffer using 50 μl of HBGA-conjugated magnetic beads with two automated extraction systems
| System | Strain | No. of positive replicates/total no. at input virus titera of:
|
||||
|---|---|---|---|---|---|---|
| 104 | 103 | 102 | 101 | 100 | ||
| iCropTheBug | GI.1 | — | 3/3 | 3/3 | 1/3 | 0/3 |
| GI.3b | — | — | 1/3 | 1/3 | 1/3 | |
| GI.13 | 3/3 | 3/3 | — | 1/3 | 0/3 | |
| GII.2 | 3/3 | 1/3 | 2/3 | 0/3 | — | |
| GII.3 | 3/3 | 3/3 | 3/3 | 1/3 | 0/3 | |
| GII.4 2002 | 3/3 | 3/3 | 1/3 | 1/3 | — | |
| GII.4 2004 | 3/3 | 2/3 | — | 2/3 | 0/3 | |
| GII.4 2006a | 3/3 | 3/3 | 2/3 | — | 0/3 | |
| GII.4 2006b | 3/3 | 2/3 | 1/3 | — | — | |
| Pathatrix | GI.1 | 3/3 | 3/3 | 3/3 | 0/3 | — |
| GI.3b | 3/3 | 3/3 | 0/3 | — | — | |
| GI.13 | — | 0/3 | 0/3 | 0/3 | — | |
| GII.2 | — | 3/3 | 2/3 | 1/3 | 0/3 | |
| GII.3 | — | — | 3/3 | 3/3 | 1/3 | |
| GII.4 2002 | 3/3 | 3/3 | 1/3 | 1/3 | — | |
| GII.4 2004 | — | — | 1/3 | 0/3 | 0/3 | |
| GII.4 2006a | 3/3 | 1/3 | 0/3 | 0/3 | — | |
| GII.4 2006b | — | 3/3 | 1/3 | 0/3 | — | |
a
The input titer was calculated for each experiment and varied due to differences in the stock sample. The quantity of input virus has been rounded to the nearest factor of 10 copies/250 ml. —, dilution not tested.