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. 2009 May 8;75(13):4573–4579. doi: 10.1128/AEM.00351-09

FIG. 3.

FIG. 3.

(A) Gene organization of YPR159W and YPR160W in S. cerevisiae (top panels) and the lager strain CMBS-33 (bottom panels). The gray regions are S. cerevisiae-like and the white regions S. bayanus-like. The coordinates for the S. cerevisiae genes on chromosome XVI are shown, and arrows indicate the direction of transcription. (B) Percentage sequence identity of the first 800 nucleotides of the CMBS-33 YPR160W gene to S. cerevisiae (gray) and S. bayanus (black) homologues. (C) Comparison of YPR160W DNA sequences of CMBS-33 (Lager), S. cerevisiae (S.c), and S. bayanus (S.b.) in the region where recombination has occurred. Nucleotides shown in black are common to all three species, those in red are identical in S. cerevisiae and CMBS-33, those in blue are identical in S. bayanus and CMBS-33, and those in pink are identical in S. cerevisiae and S. bayanus. The nine extra nucleotides at positions 450 to 459 present in CMBS and S. bayanus but absent in S. cerevisiae are shown. The in-frame stop codon in the CMBS sequence is underlined. Numbers on the right indicate the nucleotide position relative to the start codon (not shown) for the lager YPR160 gene. The full DNA sequence is shown in Fig. S1 in the supplemental material.