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Relationship between R488/435 of the individual cells of Listeria monocytogenes N53-1 and pHi. The pHi was equilibrated to pHex by incubating preparations with 70% ethanol and dissolving them at different pH values in buffer (A) or TSB medium (B). The ratio values are averages based on at least 40 single cells. The error bars indicate standard deviations.
