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Reproducibility of the SYBR green I assay for total mcrA copies conducted for 18 standard curves in three separate qPCRsa
| Plate no. | Slope | Efficiency (%) | y intercept | R2 |
|---|---|---|---|---|
| 1 | −3.416 ± 0.054 | 96.2 ± 2.1 | 38.78 ± 0.72 | 0.9954 ± 0.0037 |
| 2 | −3.470 ± 0.097 | 94.2 ± 3.7 | 39.48 ± 0.49 | 0.9964 ± 0.0030 |
| 3 | −3.464 ± 0.099 | 94.4 ± 3.9 | 40.24 ± 0.36 | 0.9974 ± 0.0069 |
| Combined | −3.451 ± 0.079 | 94.9 ± 3.1 | 39.40 ± 0.69 | 0.9962 ± 0.0030 |
Values are means ± standard deviations.
