TABLE 3.

Reproducibility of TaqMan assays targeting different methanogen groups from three standard curves from three separate qPCRs^a

Probe	Slope	Efficiency (%)	y intercept	R2
mbac-mcrA	−3.518 ± 0.141	92.4 ± 5.3	42.20 ± 0.89	0.9949 ± 0.0004
mrtA	−3.538 ± 0.064	91.7 ± 2.3	41.06 ± 0.32	0.9937 ± 0.0035
mcp	−3.487 ± 0.040	93.6 ± 1.5	42.75 ± 1.44	0.9912 ± 0.0041
msp	−3.570 ± 0.104	90.6 ± 3.7	41.66 ± 0.43	0.9930 ± 0.0040
MCR-7	−3.505 ± 0.040	92.9 ± 1.5	41.41 ± 0.26	0.9964 ± 0.0014
MCR-2a	−3.513 ± 0.077	92.6 ± 2.8	40.67 ± 0.97	0.9953 ± 0.0057
MCR-2b	−3.427 ± 0.355	95.8 ± 15.8	42.73 ± 1.47	0.9864 ± 0.0130
Fen	−3.345 ± 0.121	99.4 ± 5.2	41.24 ± 1.46	0.9930 ± 0.0043
msar	−3.477 ± 0.094	93.9 ± 3.6	43.64 ± 1.24	0.9953 ± 0.0034
msa	−3.481 ± 0.074	93.8 ± 2.8	42.45 ± 1.45	0.9977 ± 0.0016

^aValues are means ± standard deviations.